Abstract
Record the knowledge, attitudes and practices about biosafety and behavior in a critical environment of the servers of a Sterilized Material Center (SMC) in a reference hospital in infectiology. It was a prospective, descriptive study in which data collection took place through a questionnaire applied before training and another after training and an observational survey. It was evidenced that 10% of the servers in this sector had no understanding of behavior in a critical environment, 30% demonstrated in the tests that they did not know how to use the Individual and Collective Protection Equipment (PPE’s and CPE’s) correctly, and 10% were wrong. questions on high-level cleaning, disinfection and sterilization of critical articles. All reported never having participated in any training on this topic. During the observational survey, servers without PPE were found during the washing of contaminated instruments, even with the equipment in the sector. The result of the questionnaire showed that it is necessary to intensify the visits of internal audits to investigate non-conformities regarding the server and the environment and also to urge the team to adhere to the use of PPE’s / EPC’s.