Abstract
Through the cultivation of the microalgae Chlorella pyrenoidosa was used the discontinuous biotechnological process, which was obtained a microalgal biomass (dry). This was directed to the supercritical processing apparatus, in which after use it was obtained an extract. This was analyzed to verify the types (varieties) of fatty acids in a gas chromatograph and also, it was coupled to mass spectrometry. It was noted that to perform the analysis in these two devices there was a complementarity between the results of types of fatty acids.