Keywords
Monitoring
Surgery
Analgesia
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Abstract
Objective: Evaluate the effects of anesthetic protocols on ovariosalpingohysterectomy (OSH) in rats, evaluating HR, RR, Tº, induction and maintenance time. Methods: 28 Wistar rats were used, divided into 5 groups with 7 animals each: Group QX: Ketamine 80mg/Kg + Xylazine 20mg/Kg; QXF Group: Ketamine 60 mg/kg + Xylazine 8 mg/kg + Fentanyl 0.03 mg/kg; QM Group: Ketamine 70 mg/kg + Midazolam 5mg/kg; QMF Group: Ketamine 60 mg/kg + Midazolam 5mg/kg + Fentanyl 0.03 mg/kg. OSH and animal monitoring were performed. Results: Intraoperatively: In CF, QMF was the one that presented the highest values at all times, while QXF presented the lowest values, and the groups with xylazine (QX and QXF) had values below physiological values, a fact also observed in RP. Postoperatively: QMF had the highest mean and was the only one with a normal HR value, with QX being the only one with values below normal. In FR, QM had a higher average, while QXF and QX presented values below physiological levels at times. Conclusion: The QMF protocol maintained HR, RR and TºC values as close to physiological values.
References
ALBRECHT M, et al. Effects of isoflurane, ketamine-xylazine and a combination of medetomidine, midazolam and fentanyl on physiological variables continuously measured by telemetry in Wistar rats. BMC veterinary research. 2014; 10(1): 198.
BATISTA FA, et al. Animal Lab CAre. Version 1.0 [software]. Universidade do Estado do Pará. 2018.
BRASIL. Ministério da Ciência, Tecnologia e Inovações. Dispõe sobre os formulários unificados para solicitação de autorização para uso de animais em ensino ou pesquisa científica e sobre a autorização e certificação pelas Comissões de Ética no Uso de Animais - CEUAs. Resolução Normativa CONCEA Nº52, Acessado em: 19 de maio de 2021.
BRASIL. Ministério da Ciência, Tecnologia e Inovações. Atualiza o texto da Diretriz Brasileira para o Cuidado e a Utilização de Animais em Atividades de Ensino ou de Pesquisa Científica - DBCA. Resolução Normativa CONCEA Nº55. Acessado em: 5 de outubro de 2022.
CAO Y, et al. Neurofilament degradation is involved in laparotomy-induced cognitive dysfunction in aged rats. Aging (Albany NY). 2020; 12(24): 25643-57.
CÉLÈRIER E, et al. Long-lasting hyperalgesia induced by fentanyl in rats preventive effect of ketamine. Anesthesiology: The Journal of the American Society of Anesthesiologists. 2000; 92(2): 465.
CICERO L, et al. Anesthesia protocols in laboratory animals used for scientific purposes. Acta Biomed. 2018; 89(3): 337-42.
DERA-SZYMANOWSKA A, et al. Immunomodulation inhibits the development of endometriosis in rats. J Physiol Pharmacol. 2020; 71(1).
DOBLER, GH, et al. Considerações na Anestesia de Animais de Laboratório. Revista Contexto & Saúde, 2019; 19(36).
FAUSTINO-ROCHA, A, et al. O papel do rato na investigação biomédica. Revista Lusófona de Ciência e Medicina Veterinária, 2019; 10: 49-61.
FLEISCHMANN T, et al. Injection anaesthesia with fentanyl–midazolam–medetomidine in adult female mice: importance of antagonization and perioperative care. Laboratory animals. 2016; 50(4): 264-74.
FONTOURA-ANDRADE JL, et al. Effect of preconditioning and postoperative hyperbaric oxygen therapy on colonic anastomosis healing with and without ischemia in rats. Acta Cir Bras. 2020; 35(5): 202000503.
FORTEA JI, et al. Sevoflurane versus ketamine+diazepam anesthesia for assessing systemic and hepatic hemodynamics in rats with non-cirrhotic portal hypertension. PLoS One. 2020; 15(5): 0233778.
GERGYE CH, et al. A Comparison of Ketamine or Etomidate Combined with Xylazine for Intraperitoneal Anesthesia in Four Mouse Strains. J Am Assoc Lab Anim Sci. 2020; 59(5): 519-30.
GREENFIELD EA. Administering Anesthesia to Mice, Rats, and Hamsters. Cold Spring Harb Protoc. 2019; 6.
KHAJURIA DK, et al. Descrição de um novo método de ooforectomia em ratas. Revista Brasileira de Reumatologia. 2012; 52(3): 466-70.
LIPISKI M, et al. Premedication with fentanyl-midazolam improves sevoflurane anesthesia for surgical intervention in laboratory mice. Exp Biol Med (Maywood). 2017; 242(12): 1287-98.
OGUZ A, et al. Investigation of antioxidant effects of rosmarinic acid on liver, lung and kidney in rats: a biochemical and histopathological study. Folia Morphol (Warsz). 2020; 79(2): 288-95.
SARTORI AS e MELLO JRBD. Anestesia em animais de laboratório: Revisão Bibliográfica. Vet Foco. 2018; 19-28.
SEABRA DI. Refinamento das técnicas de anestesia injetável em hamsters. Dissertação (Mestrado em Patologia Experimental e Comparada) – Faculdade de Medicina Veterinária e Zootecnia. Universidade de São Paulo, São Paulo, 2019; 130.
SOPHOCLEOUS A e IDRIS AI. Ovariectomy/Orchiectomy in Rodents. Methods Mol Biol. 2019; 1914: 261-7.
SOUZA VR, et al. Description of Ovariectomy Protocol in Mice. Methods Mol Biol. 2019; 1916: 303-9.
TASHIRO M, et al. Duration of thermal support for preventing hypothermia induced by anesthesia with medetomidine-midazolam-butorphanol in mice. J Vet Med Sci. 2020; 82(12): 1757-62.
TSUKAMOTO A, et al. The validity of anesthetic protocols for the surgical procedure of castration in rats. Experimental animals. 2018; 18-0003.