Keywords
Alcohol-related diseases
Patents
Technological monitoring
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Abstract
Objective: To collect and analyze patent documents related to zebrafish as an animal model for alcohol-related diseases (liver diseases, heart diseases, cancer, and Fetal Alcohol Spectrum Disorders - FASD) based on WHO definitions. Methods: A descriptive, quantitative, and qualitative technological monitoring study was conducted using national and international patent databases. The documents were analyzed by alcohol-affected organ, animal model, legal status, type of applicant, and temporal and geographic distributions, presented by figures and tables. Results: Out of 2,166 patents identified, 13 met the inclusion criteria. Of these, 54% were in the application stage, and 46% had been granted. Most patents were filed by companies, with dominant IPC codes A61K (medical preparations) and A61P (therapeutic activities). Liver diseases accounted for 71.43% of the patents, followed by FASD (21.43%) and liver cancer (7.14%). Zebrafish was the primary model in 11 patents; two used rats but mentioned zebrafish as an alternative. Conclusion: Zebrafish use in alcohol-related disease research is emerging, primarily focused on liver diseases.
References
ALVES RL, et al. Investigating the role of alcohol consumption during pregnancy in public health systems: Challenges in Brazil. Revista Brasileira de Enfermagem, 2023; 76(2): 219-227.
BRAGA APA, et al. The Zebrafish as an Alternative Animal Model for Ecotoxicological Research and Testing. Brazilian Archives of Biology and Technology, 2024; 67: 24220968.
CADENA PG, et al. Folic acid reduces the ethanol-induced morphological and behavioral defects in embryonic and larval zebrafish (Danio rerio) as a model for fetal alcohol spectrum disorder (FASD). Reproduction Toxicology, 2020; 96: 249-257.
CADENA PG, et al. Protective effects of quercetin, polydatin, and folic acid and their mixtures in zebrafish (Danio rerio) fetal alcohol spectrum disorder model. Neurotoxicology and Teratology, 2020; 82: 106928.
CANEDO LP. Protocolo experimental em laboratório de ensino prático para avaliação de toxicidade aguda utilizando embriões de zebrafish (Danio rerio). Revista de Patologia Tropical, 2023; 52: 102-116.
CHATTERJEE D, et al. Chronic and acute alcohol administration induced neurochemical changes in the brain: comparison of distinct zebrafish populations. Amino Acids, 2014; 46(4): 921-30.
GINÈS P, et al. Liver cirrhosis. Lancet, 2021; 398(10308): 1359-1376.
HOWE K, et al. The zebrafish reference genome sequence and its relationship to the human genome. Nature, 2013; 496(7446): 498-503.
LARA-BERTRAND CH, et al. A framework for alcohol use disorder risk prediction using multi-modal patient data. World Psychiatry Institute, 2023; 55: 202-217.
LI S, et al. Effect of a brief intervention with small financial incentives on alcohol consumption in China: study protocol for a randomized controlled trial. BJM Open, 2022; 12(5): 56550.
MANIKANDAN P, et al. Ethanol effects on early developmental stages studied using the zebrafish. Biomedicines, 2022; 10(10): 2555.
MARRS JA, et al. Zebrafish fetal alcohol syndrome model: effects of ethanol are rescued by retinoic acid supplement et al., Alcohol, 2010; 44: 707-715.
MILEWSKI CS, et al. Prospecção tecnológica de patentes do uso do zebrafish em substituição aos animais de laboratório. Cadernos de Prospecção, 2024; 17(2): 603–620.
PAHO. PAN AMERICAN HEALTH ORGANIZATION. Assessment of Fetal Alcohol Spectrum Disorders. 2020. ISBN: 978-92-75-12224-2.
PAHO. PAN AMERICAN HEALTH ORGANIZATION. Document PAHO/NMH/MH/22-0020. 2022.
POPOVA S, et al. Prevalence of Fetal Alcohol Spectrum Disorder among Special Subpopulations: A Systematic Review and Meta-Analysis. Addiction, 2019; 112(11): 1929-1946.
SILVA MLSC, et al. Monitoramento tecnológico de terapêuticas utilizadas para prevenção e tratamento da síndrome alcoólica fetal do período pré ao pós-natal, Revista Eletrônica Acervo Saúde, 2022; 15(7): 10460.
SYED J, et al. A perspective on psychedelic teratogenicity: the utility of zebrafish models. Trends in Pharmacological Sciences, 2023; 44(9): 765-780.
WHO. WORLD HEALTH ORGANIZATION. Alcohol. Available at: https ://www.who.int/news-room/fact-sheets/detail/alcohol. Accessed on: September 10, 2024.
WHO. WORLD HEALTH ORGANIZATION. Global Status Report on Alcohol and Health, 2018.