Keywords
Microbiota
Antimicrobial resistance
Potamotrygoninae
Amazon
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Abstract
Objective: To study the community and the antimicrobial resistance profile of bacteria isolated from the skin tissue of rays from the Potamotrygoninae subfamily, collected from the Amazonas and Tapajós rivers. Methods: After capturing four rays, two females and two males, from two locations in the Amazon, a swab soaked in tryptone soy broth was used to collect material from the dorsal region of the skin and mucus of the animals. The collected material underwent serial dilution to 10-5, and the isolates were biochemically identified. The Kirby-Bauer method was used to assess the antimicrobial resistance profile against eight antimicrobials, and the results were evaluated according to BrCAST. Results: Seventy-six bacterial strains were isolated, with Corynebacterium spp. and Bacillus spp. predominating, and 61% of the strains were resistant to at least one antibiotic, with the most common being oxacillin and penicillin. Conclusions: This study was the first to isolate bacteria from the Corynebacterium genus in rays, with the highest resistance to oxacillin and penicillin within the penicillin class. From the One Health perspective, the use of fish and freshwater ecosystems to monitor the spread of resistance genes is of interest, considering the close relationship between Amazonian populations and aquatic ecosystems and the health of these animals.
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