Abstract
Objective: To verify the occurrence of pathogenic microorganisms in lesions of patients with diabetic foot, to identify, to determine the incidence of bacterial species and to characterize the susceptibility profile. Methods: The research was approved by the Research Ethics Committee of the Center for Higher Studies of Caxias-Ma (CESC-UEMA) with the opinion of No. 809,407. Clinical specimens were obtained from patients attended at the General Hospital of the municipality. Samples were collected using swab and seeded in the media agar and blood agar and methylene blue eosin agar. Sows were incubated in a BOD oven at 37 ° C for 24 h. The microorganisms were identified by means of biochemical tests, in Rugai and coagulase test. The susceptibility profile was determined by the Kirby & Bauer method. Results: 30 patients with diagnosis of diabetic foot admission were included in the study. Most were aged between 50-70 years. The duration of diabetes in 63.8% of the overall sample was between 2 and 10 years. Of the patients used in the research, 66.7% had moderate infection and 33.3% had severe infection. Of the microorganisms isolated and identified to the prevalence was greater of enterobacteria, especially for the species Klebsiella spp. Regarding antimicrobial susceptibility profiles, Pseudomonas aeruginosa and Klebsiella spp presented higher resistance to several antibiotics. Conclusion: Ulcers of patients with diabetic foot are potential sites for contamination by pathogenic microorganisms. The highest prevalence of isolated and identified microorganisms was of enterobacteria, especially Klebsiella spp.