Palabras clave
Peces
Modelo animal
Epibolia
Efecto teratogénico
Cómo citar
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Resumen
Objetivo: Proponer al pez cebra (Danio rerio) como modelo animal de intoxicación fetal por exposición a deltametrina (DM) evaluando la concentración y el tiempo, considerando que las primeras 24 h de este modelo equivalen al primer trimestre del desarrollo fetal humano. Métodos: Los embriones fueron expuestos a 100 - 1000 µg/L (DM). Se realizaron tres experimentos donde se evaluó la epibolia a las 8 h y los efectos teratogénicos y la mortalidad a las 22 y 46 h de exposición. Resultados: Se observó un retraso en el epibolismo (61,41, 55,05 y 50,87%) en los grupos expuestos a DM de forma dosis-dependiente. La exposición a DM durante 22 h fue suficiente para inducir efectos teratogénicos en embriones como edema pericárdico y del saco vitelino y deformación de la columna y la cola. La exposición durante 46 h también indujo los efectos informados anteriormente, pero causó una mayor mortalidad animal. La exposición a 1000 µg/L provocó la mortalidad del 100% de los animales. Conclusión: Concluimos que la concentración de 500 µg/L y 22 h de exposición produjo cambios en la epibolia y efectos teratogénicos que pueden ser evaluados durante el desarrollo embrionario y resultó ser el mejor para ser utilizado en futuros estudios en investigación en salud.
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