Resumen
Objetivo: Evaluar la eficacia de desinfectantes naturales, como aceites esenciales y extractos vegetales, en la reducción de Escherichia coli O157:H7 y Salmonella spp. en hojas de lechuga. Métodos: Se realizó una revisión integrativa de la literatura. Se seleccionaron artículos publicados hasta abril de 2025 en las bases PubMed, ScienceDirect, SciELO y EBSCO. Se incluyeron estudios que analizaron la acción antimicrobiana de aceites esenciales o extractos vegetales en variedades de lechuga contaminadas con E. coli O157:H7 y Salmonella spp. Resultados: De 234 estudios, siete cumplieron los criterios. Los desinfectantes naturales lograron una reducción microbiana promedio de 1,55 log UFC/g, superior al agua (0,5 log UFC/g; p < 0,001) y similar al cloro (1,95 log UFC/g; p = 0,274). El aceite esencial de tomillo destacó con reducciones mayores a 3 log UFC/g. No se observaron diferencias significativas entre microorganismos ni tipos de desinfectantes. Concentración, tiempo de tratamiento y secado no afectaron la eficacia. Consideraciones finales: Los desinfectantes naturales son una alternativa prometedora, especialmente cuando se combinan con tecnologías como la microencapsulación. La falta de estandarización metodológica requiere más estudios para garantizar su seguridad y aplicación comercial.
Referencias
ALLENDE A, et al. Role of commercial sanitizers and washing systems on epiphytic microorganisms and sensory quality of fresh-cut escarole and lettuce. Postharvest Biology and Technology. 2008;49(1):155-163.
BHATIA R, et al. Microbial risks associated with ready-to-eat fresh produce (RTEFP): A focus on temperate climatic conditions. Food Control. 2024;155:114041.
CHEN JC, et al. Reoccurring Escherichia coli O157:H7 strain linked to leafy greens–associated outbreaks, 2016–2019. Emerging Infectious Diseases. 2023;29(9):1895.
EHUWA O, et al. Salmonella, food safety and food handling practices. Foods. 2021;10(5):907.
ELIAS S, et al. Salmonella spp. and Escherichia coli O157:H7 prevalence and levels on lettuce: A systematic review and meta-analysis. Food Microbiology. 2019;84:103217.
ELPERS L, et al. Factors required for adhesion of Salmonella enterica serovar Typhimurium to Lactuca sativa (lettuce). Microbiology Spectrum. 2023;11(1):e03436-22.
FAN X, SOKORAI KJ. Formation of trichloromethane in chlorinated water and fresh-cut produce and as a result of reaction with citric acid. Postharvest Biology and Technology. 2015;109:65-72.
FERNANDES AP, et al. Encapsulation of essential oils in nanoemulsions: Balancing antimicrobial effect and sensory acceptability. Journal of Food Engineering. 2024;315:111232.
GÜNDÜZ GT, et al. Antimicrobial activity of oregano oil on iceberg lettuce with different attachment conditions. Journal of Food Science. 2012;77(7):M412-M415.
GÜNDÜZ GT, et al. Efficacy of myrtle oil against Salmonella Typhimurium on fresh produce. International Journal of Food Microbiology. 2009;130(2):147-150.
GÜNDÜZ GT, et al. Efficacy of oregano oil in the inactivation of Salmonella Typhimurium on lettuce. Food Control. 2010;21(4):513-517.
JENSEN DA, et al. Cross-contamination of Escherichia coli O157:H7 between lettuce and wash water during home-scale washing. Food Microbiology. 2015;46:428–433.
JOSHI K, et al. Novel disinfectants for fresh produce. Food Control. 2013;34(1):100–111.
KANG J, SONG KB. Antimicrobial activity of honeybush (Cyclopia intermedia) ethanol extract against foodborne pathogens and its application in washing fresh cut Swiss chard. Food Control. 2021;121:107674.
KIM SY, et al. Antimicrobial activity of plant extracts against Salmonella Typhimurium, Escherichia coli O157:H7, and Listeria monocytogenes on fresh lettuce. Food Control. 2011.
KOUKKIDIS G, et al. Salad leaf juices enhance Salmonella growth, colonization of fresh produce, and virulence. Applied and Environmental Microbiology. 2017;83(1):e02416-16.
LEE CH, et al. Inactivation of Listeria monocytogenes and Escherichia coli O157:H7 inoculated on fresh-cut romaine lettuce by peanut skin extract/benzethonium chloride emulsion washing. Food Control. 2021;119:107479.
LIMA CM, SOUZA E. A systematic quantitative analysis of the published literature on the efficacy of essential oils as sanitizers in fresh leafy vegetables. Critical Reviews in Food Science and Nutrition. 2021;61(14):2326-2339.
LOAN HNB, et al. 3-Chlorotyrosine formation in ready-to-eat vegetables due to hypochlorite treatment and its dietary exposure and risk assessment. Food Research International. 2016;90:186–197.
LOZANO IM, et al. Nutritional value of commercial and traditional lettuce (Lactuca sativa L.) and wild relatives: Vitamin C and anthocyanin content. Food Chemistry. 2021;359:129864.
MARTÍNEZ FJA, et al. Antibacterial plant compounds, extracts and essential oils: An updated review on their effects and putative mechanisms of action. Phytomedicine. 2021;90:153626.
MULAOSMANOVIC E, et al. Size matters: Biological and food safety relevance of leaf damage for colonization of Escherichia coli O157:H7 gfp+. Frontiers in Microbiology. 2021;11:608086.
OWADE JO, et al. A review of conditions influencing fate of Shiga toxin‐producing Escherichia coli O157:H7 in leafy greens. Comprehensive Reviews in Food Science and Food Safety. 2024;23(5):e70013.
PEREIRA R, et al. Decontamination of minimally-processed fresh lettuce using reuterin produced by Lactobacillus reuteri. Frontiers in Microbiology. 2018;9:1421.
QIN YM, et al. Deep learning for sustainable agriculture: A systematic review on applications in lettuce cultivation. Sustainability. 2025;17(7):3190.
SILVA BN, et al. Meta-analysis of the incidence of foodborne pathogens in vegetables and fruits from retail establishments in Europe. Current Opinion in Food Science. 2017;18:70–77.
SILVA LP, et al. Meta analysis of the effects of sanitizing treatments on Salmonella, Escherichia coli O157:H7, and Listeria monocytogenes inactivation in fresh produce. Applied and Environmental Microbiology. 2015;81:8008–8021.
SINGH N, et al. Effect of inoculation and washing methods on the efficacy of different sanitizers against Escherichia coli O157:H7 on lettuce. Food Microbiology. 2002;19(2-3):183-193.
SSEMANDA JN, et al. Reduction of microbial counts during kitchen-scale washing and sanitization of salad vegetables. Food Control. 2018;85:495–503.
TARIQ S, et al. A comprehensive review of the antibacterial, antifungal and antiviral potential of essential oils and their chemical constituents against drug-resistant microbial pathogens. Microbial Pathogenesis. 2019;134:103580.
THOMAS GA, et al. From field to plate: How do bacterial enteric pathogens interact with ready-to-eat fruit and vegetables, causing disease outbreaks? Food Microbiology. 2024;117:104389.
VIDAKOVIĆ V, et al. Qualitative profiling, antioxidant and antimicrobial activities of polar and nonpolar basil extracts. Foods. 2024;13(18):2993.
WHO. Healthy diet. World Health Organization. 2020. Disponível em: https://www.who.int/news-room/fact-sheets/detail/healthy-diet. Acessado em: 11 de fevereiro de 2025.
YU K, et al. Effect of chitosan coatings with cinnamon essential oil on postharvest quality of mangoes. Foods. 2021;10(12):3003.
ŽAGAR T, et al. Using subcritical water to obtain polyphenol-rich extracts with antimicrobial properties. Antibiotics. 2024;13(4):334.
ZAMAN S, et al. Use of non-chlorine sanitizers in improving quality and safety of marketed fresh salad vegetables. Processes. 2024;12(5):1011.
ZAMUZ S, et al. The role of phenolic compounds against Listeria monocytogenes in food: A review. Trends in Food Science & Technology. 2021;110:385-392.
