Palabras clave
Derivado de pirazol
Actividad antimicrobiana
CMI
Cómo citar
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Resumen
Objetivo: Analizar la actividad antimicrobiana de una chalcona tiofénica y un derivado de pirazol sintetizados a través de rutas químicas específicas. Métodos: Este es un estudio experimental in vitro. La chalcona tiofénica (GA-01) se sintetizó por condensación aldólica y su derivado de pirazol (GA-04) por ciclocondensación con tiosemicarbazida. Los compuestos se caracterizaron por RMN de ¹H y ¹³C. La actividad antimicrobiana se evaluó determinando la concentración mínima inhibitoria (CMI) contra Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus y Candida albicans, utilizando el método de microdilución en placa. Para investigar el mecanismo de acción, se realizó un ensayo con sorbitol 0,8 M, evaluando el impacto osmoprotector en la eficacia de los compuestos. Resultados: La chalcona GA-01 tuvo una CMI de 128 μg/mL para todas las cepas. El GA-04 mostró mayor actividad, con una CMI de 64 μg/mL para bacterias y 4 μg/mL para C. albicans. En el ensayo con sorbitol, se observó un aumento de la CMI para ambos compuestos, lo que sugiere una acción sobre la pared celular microbiana. Conclusión: Los compuestos sintetizados mostraron un potencial antimicrobiano prometedor, con énfasis en el nuevo derivado de pirazol (GA-04), que demostró una mayor eficacia. Los resultados indican que estos compuestos son prototipos para el desarrollo de nuevos agentes antimicrobianos.
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