Keywords
Uterine cervix
p16
Ck7
Biomarkers
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Abstract
Objective: To investigate potential molecular markers as prognostic factors in immature squamous metaplasia (ISM) in the cervix and to evaluate the clinical significance of finding typical and atypical ISM in the cervical epithelium of the patient. Methods: A total of 79 cases were selected in a retrospective cross-sectional study (2011-2019). Women with typical or atypical ISM were evaluated according to sociodemographic, cytological, and colposcopy criteria. The biopsy samples were evaluated by anatomopathological and immunohistochemical analysis, in which the expression of P16INK4a (p16) and Ck7 was investigated. Results: Cytological analysis showed a higher frequency of atypical squamous cells of undetermined significance with high-grade lesions. Regarding colposcopy analysis, both groups presented similar results. The negativity of p16 and Ck7 expression was associated with a favorable outcome. However, no correlations were found between the expression of p16 and Ck7 and typical or atypical ISM. In addition, a kappa value of 0.087 was obtained, confirming the significant interobserver variability of the study. Conclusion: The absence of p16 and Ck7 can be used as biomarkers of favorable prognosis, but they did not show correlations with classifications of typical or atypical ISM in the cervix. Therefore, other effective methodologies for screening this patient profile should be further investigated.
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