Modelo de zebrafish (Danio rerio) para intoxicação fetal por deltametrina
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Resumo
Objetivo: Propor o zebrafish (Danio rerio) como um modelo animal de intoxicação fetal pela exposição a deltametrina (DM) avaliando a concentração e tempo, considerando que as primeiras 24 h deste modelo equivalem ao primeiro trimestre de desenvolvimento fetal humano. Métodos: Embriões foram expostos as concentrações 100 - 1000 µg/L (DM). Foram realizados três experimentos onde a epibolia foi avaliada a 8 h e os efeitos teratogênicos e mortalidade foram avaliados após 22 e 46 h de exposição. Resultados: Foi observado atraso na epibolia (61,41, 55,05 e 50,87%) nos grupos expostos a DM de forma dose dependente. A exposição a DM por 22 h foi suficiente para induzir efeitos teratogênicos nos embriões como edemas de pericárdio e saco vitelino e deformação de coluna e cauda. Já a exposição por 46 h também induziu os efeitos relatados acima, porem ocasionou maior mortalidade dos animais. A exposição a 1000 µg/L ocasionou a mortalidade de 100% dos animais. Conclusão: Concluímos que a concentração de 500 µg/L e 22 h de exposição produziu alterações na epibolia e efeitos teratogênicos que podem ser avaliados durante o desenvolvimento embrionário e se mostrou a melhor para ser usada em estudos futuros em pesquisa de saúde.
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