Perfil imunohematológico e marcadores informativos de ancestralidade em indivíduos com COVID-19
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Apr 25, 2025
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Resumo
Objetivo: Avaliar os perfis hematológicos e linfocitários de indivíduos com COVID-19, bem como, identificar o perfil ancestral por meio de Marcadores Informativos de Ancestralidade de duas cidades da região Nordeste do Brasil. Métodos: Foram analisados os dados de 58 indivíduos com COVID-19 que apresentavam condições clínicas leves (39) e graves (19). O perfil linfocitário foi identificado por imunofenotipagem e genotipagem via análise de PCR e qPCR. Os dados foram analisados por estatística descritiva usando o software IBM SPSS Statistics. O estudo foi aprovado pelo Comitê de Ética em Pesquisa (ICS-UFBA). Resultados: Os indivíduos do grupo grave apresentaram aumento de leucócitos totais e neutrófilos, diminuição de eritrócitos, hemoglobina, hematócrito e de linfócitos em comparação aos indivíduos do grupo leve. Assim como, o grupo grave apresentou diminuição nos perfis celulares de células TCD4+, TCD8+ e linfócitos B. Em relação ao perfil ancestral, ambos os grupos apresentaram maior perfil genômico ancestral europeu. No entanto, o grupo grave apresentou maior percentual de ancestralidade africana em comparação ao grupo leve. Conclusão: A ancestralidade não foi associada à gravidade, mas os resultados correspondem às descobertas de que as respostas imunes dos indivíduos desempenha um papel crucial na COVID-19.
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Como Citar
AlmeidaG. B. de, PalmaT. F. B., BarbosaD. de J., TorresA. J. L., ConceiçãoR. R., MoraesI. R. B. de, TrindadeS. C., FortunaV. A., SimionatoS., & MarchioroS. B. (2025). Perfil imunohematológico e marcadores informativos de ancestralidade em indivíduos com COVID-19. Revista Eletrônica Acervo Saúde, 25, e18486. https://doi.org/10.25248/reas.e18486.2025
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29. SANTOS MPAD, et al. População negra e Covid-19: reflexões sobre racismo e saúde. EstudosAvançados. 2020; 34(99): 225–44.
30. SHELTON JF, et al. Trans-ancestry analysis reveals genetic and nongenetic associations with COVID-19 susceptibility and severity. Nature Genetics. 2021; 53(6): 801-808.
31. SHRIVER MD, et al. Ethnic-affiliation estimation by use of population-specific DNA markers. American Journal of Human Genetics. 1997; 60(4): 957–64.
32. SONG JW, et al. Immunological and inflammatory profiles in mild and severe cases of COVID-19. Nature Communications. 2020; 11(1): 3410.
33. SUN S, et al. Abnormalities of peripheral blood system in patients with COVID-19 in Wenzhou, China. ClinicaChimicaActa; International Journal of Clinical Chemistry. 2020; 507: 174-180.
34. TAO Z, et al. Anemia is associated with severe illness in COVID‐19: A retrospective cohort study. Journal of Medical Virology. 2020; 93(3): 1478–88.
35. TERPOS E, et al. Hematological findings and complications of COVID ‐19. American Journal of Hematology. 2020; 95(7): 834-847.
36. WANG J, et al. Excessive Neutrophils and Neutrophil Extracellular Traps in COVID-19. Frontiers in Immunology. 2020; 11: 2063.
37. WANG L, et al. Coronavirus disease 2019 in elderly patients: Characteristics and prognostic factors based on 4-week follow-up. Journal of Infection. 2020; 80(6): 639-645.
38. WESTMEIER J, et al. Impaired Cytotoxic CD8+ T Cell Response in Elderly COVID-19 Patient. mBio. 2020; 11(6): 2805-20.
39. WOOL GD e MILLER JL. The impact of COVID-19 disease on platelets and coagulation. Pathobiology. 2020; 88(1): 1–13.
40. ZHOU F, et al. Clinical course and risk factors for mortality of adult inpatients with COVID-19 in Wuhan, China: a retrospective cohort study. The Lancet. 2020; 395(10229): 1054-1062.
2. AL-ABDI S e AL-AAMRI M. G6PD deficiency in the COVID-19 pandemic: Ghost within Ghost. Hematology/Oncology and Stem Cell Therapy. 2020; 14(1): 84-85.
3. AZKUR AK, et al. Immune response to SARS‐CoV‐2 and mechanisms of immunopathological changes in COVID‐19. Allergy. 2020; 75 (7): 1564–81.
4. BORGES GM e CRESPO CD. Aspectos demográficos e socioeconômicos dos adultos brasileiros e a COVID-19: uma análise dos grupos de risco a partir da Pesquisa Nacional de Saúde, 2013. Cadernos de SaúdePública. 2020; 36(10): 141020.
5. CAVALCANTE LN, et al. Genetic ancestry analysis in non-alcoholic fatty liver disease patients from Brazil and Portugal. World Journal of Hepatology. 2015; 7(10): 1433-1438.
6. CODD AS, et al. Neutrophilia, lymphopenia and myeloid dysfunction: a living review of the quantitative changes to innate and adaptive immune cells which define COVID-19 pathology. Oxford Open Immunology. 2021; 2(1): 16.
7. COZIER YC, et al. Lower serum 25(OH)D levels associated with higher risk of COVID-19 infection in U.S. Black women. PLOS ONE. 2021; 16(7): 255132.
8. DU RH, et al. Predictors of mortality for patients with COVID-19 pneumonia caused by SARS-CoV-2: a prospective cohort study. European Respiratory Journal. 2020; 55(5): 2000524.
9. FARSHBAFNADI M, et al. Aging & COVID-19 susceptibility, disease severity, and clinical outcomes: The role of entangled risk factors. Experimental Gerontology. 2021; 154(15): 111507.
10. FERDINAND K, et al. Contemporary and Future Concepts on Hypertension in African Americans: COVID-19 and Beyond. Journal of the National Medical Association. 2020; 112(3): 315–23.
11. FERREIRA RBS e CAMARGO CL. Vulnerabilidade da população negra brasileira frente à evolução da pandemia por COVID-19. RevistaCuidarte. 2021; 12(2): 1-12.
12. FU J, et al. The clinical implication of dynamic neutrophil to lymphocyte ratio and D-dimer in COVID-19: A retrospective study in Suzhou China. Thrombosis Research. 2020; 192: 3–8.
13. GHOBADI H, et al. Role of leukocytes and systemic inflammation indexes (NLR, PLR, MLP, dNLR, NLPR, AISI, SIR-I, and SII) on admission predicts in-hospital mortality in non-elderly and elderly COVID-19 patients. Frontiers in Medicine. 2022; 9: 916453.
14. HU B, et al. Characteristics of SARS-CoV-2 and COVID-19. Nature Reviews Microbiology. 2020; 20(5): 315.
15. IBGE. Censo 2010. Govbr 2010. https ://cidades.ibge.gov.br/brasil/ba/salvador/pesquisa/23/25359. Acess: April 2024.
16. JAIN SK, et al. The potential link between inherited G6PD deficiency, oxidative stress, and vitamin D deficiency and the racial inequities in mortality associated with COVID-19. Free Radical Biology and Medicine. 2020; 161: 84–91.
17. JESENAK M, et al. Immune Parameters and COVID-19 Infection – Associations With Clinical Severity and Disease Prognosis. Frontiers in Cellular and Infection Microbiology. 2020; 10: 364.
18. JING Y, et al. SARS-CoV-2 infection causes immunodeficiency in recovered patients by downregulating CD19 expression in B cells via enhancing B-cell metabolism. Signal Transduction and Targeted Therapy. 2021; 6(1): 345.
19. KARIMI SHAHRI M, et al. COVID‐19 and hematology findings based on the current evidences: A puzzle with many missing pieces. International Journal of Laboratory Hematology. 2020; 43(2): 160–8.
20. MACKEY K, et al. Racial and Ethnic Disparities in COVID-19–Related Infections, Hospitalizations, and Deaths. Annals of Internal Medicine. 2021; 174 (3): 362-373.
21. MERAD M, et al. The immunology and immunopathology of COVID-19. Science. 2022; 375(6585): 1122–7.
22. MOSS P. The T cell immune response against SARS-CoV-2. Nature Immunology. 2022; 23(2): 186–93.
23. MS. MINISTÉRIO DA SAÚDE. Coronavírus Brasil. Covidsaudegovbr 2024. https ://covid.saude.gov.br/ Acess: July, 2024.
24. NORIN AJ, et al. HLA B53 is associated with a poor outcome in black COVID-19 patients. HumanImmunology. 2021; 82(10): 713-718.
25. PENA J. Razões para banir o conceito de raça da medicina brasileira. HistoriaCienciasSaude-manguinhos. 2005; 12(2): 321–46.
26. PENA SDJ, et al. Genetic admixture in Brazil. American Journal of Medical Genetics Part C: Seminars in Medical Genetics. 2020; 184(4): 928-938.
27. PRICE-HAYWOOD EG, et al. Hospitalization and Mortality among Black Patients and White Patients with Covid-19. New England Journal of Medicine. 2020; 382(26): 2534-2543.
28. QUEIROZ EM, et al. Genetic composition of a Brazilian population: the footprint of the Gold Cycle. Geneticsand molecular research: GMR. 2013; 12(4): 5124-5133.
29. SANTOS MPAD, et al. População negra e Covid-19: reflexões sobre racismo e saúde. EstudosAvançados. 2020; 34(99): 225–44.
30. SHELTON JF, et al. Trans-ancestry analysis reveals genetic and nongenetic associations with COVID-19 susceptibility and severity. Nature Genetics. 2021; 53(6): 801-808.
31. SHRIVER MD, et al. Ethnic-affiliation estimation by use of population-specific DNA markers. American Journal of Human Genetics. 1997; 60(4): 957–64.
32. SONG JW, et al. Immunological and inflammatory profiles in mild and severe cases of COVID-19. Nature Communications. 2020; 11(1): 3410.
33. SUN S, et al. Abnormalities of peripheral blood system in patients with COVID-19 in Wenzhou, China. ClinicaChimicaActa; International Journal of Clinical Chemistry. 2020; 507: 174-180.
34. TAO Z, et al. Anemia is associated with severe illness in COVID‐19: A retrospective cohort study. Journal of Medical Virology. 2020; 93(3): 1478–88.
35. TERPOS E, et al. Hematological findings and complications of COVID ‐19. American Journal of Hematology. 2020; 95(7): 834-847.
36. WANG J, et al. Excessive Neutrophils and Neutrophil Extracellular Traps in COVID-19. Frontiers in Immunology. 2020; 11: 2063.
37. WANG L, et al. Coronavirus disease 2019 in elderly patients: Characteristics and prognostic factors based on 4-week follow-up. Journal of Infection. 2020; 80(6): 639-645.
38. WESTMEIER J, et al. Impaired Cytotoxic CD8+ T Cell Response in Elderly COVID-19 Patient. mBio. 2020; 11(6): 2805-20.
39. WOOL GD e MILLER JL. The impact of COVID-19 disease on platelets and coagulation. Pathobiology. 2020; 88(1): 1–13.
40. ZHOU F, et al. Clinical course and risk factors for mortality of adult inpatients with COVID-19 in Wuhan, China: a retrospective cohort study. The Lancet. 2020; 395(10229): 1054-1062.