Atividade antibiofilme dos óleos essenciais de Melaleuca alternifólia e Mentha piperita frente Aeromonas spp. isoladas do peixe amazônico Tambaqui (Colossoma macropomum)
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May 22, 2025
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Objetivo: Avaliar acapacidade de formação de biofilme, como característica de virulência das cepas de Aeromonas spp. previamente isoladas do Tambaqui e a atividade antibiofilme dos óleos essenciais de Melaleuca alternifoliae Mentha piperita. Métodos: Cepas de Aeromonas hydrophila, veronii, caviae e dhakensis, anteriormente preservadas em óleo mineral, foram reativadas e preservadas em Ágar Nutriente. Para avaliação dos biofilmes, foi feita a diluição das colônias, padronização de células no espectrofotômetro e distribuição nas placas de 96 poços. Foi usado Tween 80 a 1% como diluente dos óleos e usado a CMI dos óleos na diluição seriada. Após 24h em estufa, foram feitas as lavagens das placas, fixação dos poços e coloração. As cepas foram classificadas de acordo com a capacidade de aderência. Resultados: Cepas de A. veroniie A. caviae não apresentaram aderência. As demais cepas de A. dhakensis, A. caviaee A. hydrophila foram classificadas como “fracamente aderente, 2 das 4 cepas de A. hydrophila se classificaram como “moderadamente aderente”. Conclusão: Os óleos essenciais de Melaleuca alternifólia e Mentha piperita apresentaram atividade antibiofilme. O óleo de Melaleuca alternifólia teve uma melhor atividade sobre as cepas de A. dhakensis tendo 78% de inibição em uma das cepas.
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SilvaP. P. da, LimaG. M. de S., TenórioF. das C. A. M., TenórioB. M., SilvaR. C. da, CavalcantiM. C. M., LiraY. A. C., AlvesR. N. da S., MarquesD. S. C., & CarvalhoE. V. M. M. de. (2025). Atividade antibiofilme dos óleos essenciais de Melaleuca alternifólia e Mentha piperita frente Aeromonas spp. isoladas do peixe amazônico Tambaqui (Colossoma macropomum). Revista Eletrônica Acervo Saúde, 25(5), e20117. https://doi.org/10.25248/reas.e20117.2025
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Referências
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33. MOGOSAN C, et al. A comparative analysis of the Chemical composition, anti-inflammatory, and antinociceptive effects of the essential oils from thre espécies of Menth acultivated in Romania. Molecules, 2017; 22(2): 263.
34. PESSOA RBG, et al. Aeromonas and human health disorders: Clinical approaches. Frontiers in microbiology, 2022; 13: 868890.
35. PESSOA RBG, et al. Fluorescent nanoprobes based on quantum dots conjugated to Cramollto assess surface carbo hydrates of Aeromonas spp. Biochimica et Biophysica Acta (BBA)-General Subjects, 2023; 1867(7): 130373.
36. PESSOA RBG, et al. Molecular characterization and evaluation of virulence traits of Aeromonas spp. Isolated from the tambaqui fish (Colossoma macropomum). Microbial pathogenesis, 2020; 147: 104273.
37. PESSOA RBG, et al. The genus Aeromonas: A general approach. Microbial Pathogenesis, [s.l.], 2019; 130: 81-94.
38. RHIND J. Essential Oils: A Comprehensive Handbook for Aromatic Therapy. Singing Dragon, 2020.
39. SAHA D e PAL J. In vitro antibiotic susceptibility of bactéria isolated from EUS‐affected fishes in India. Letters in Applied Microbiology, 2002; 34(5): 311-316.
40. SOUSA RGC, et al. Avaliação do ganho do peso de tambaqui cultivado com diferentes taxas de proteínas na alimentação. Biota Amazônia (Biote Amazonie, Biota Amazonia, Amazonian Biota), 2016; 6(1): 40-45.
41. STEPANOVIĆ S, et al. A modified microtiter-platetest for quantification of staphylococcal biofilm formation. Journal of microbiological methods, 2000; 40(2): 175-179.
42. STRINGARO A, et al. Antioxidant, Antifungal, Antibiofilm, and Cytotoxic Activities of Mentha spp. Essential Oils. Medicines, 2018; 5(4): 112.
43. TAVARES-DIAS M e MARIANOWS. Aquicultura no Brasil: novas perspectivas. São Carlos: Pedro & João Editores, 2015.
2. BAKKALI F, et al. Biological effects of essential oils– A review. Food And Chemical Toxicology, [s.l.], 2008; 46(2): 446-475.
3. BALLICK M e COX P. Plants, people and culture: the Science of ethnobotany. New York. Scientific American Library, 1997.
4. BARONYG, et al. New hosts and genetic diversity of flavo bacterium columnare isolated from Brazilian native species and Niletilapia. Diseases of Aquatic Organisms, 2015; 117(1): 1-11.
5. BOROTOVÁ P, et al. Chemical and biological characterization of Melaleuca alternifólia essential oil. Plants, 2022; 11(4): 558.
6. BOWER CK e DAESCHEL MA. Resistance responses of microorganisms in food environments. International Journal Of Food Microbiology, [s.l.], 1999; 50(1-2): 33-44.
7. CAMELE I, et al. Chemical composition and antimicrobial Properties of Mentha × piperita cv. Kristinka essential oil. Plants, 2021; 10(8): 1567.
8. CARVALHO EVMM, et al. Physiological and biotechnological approaches of the amazonian tambaqui fish (Colossoma macropomum). Marine biology series. Nova Science, New York, 2014; 50.
9. CLUTTERBUCKAl, et al. Bio films and their relevance to veterinary medicine. Veterinary Microbiology, [s.l.], 2007; 121(1-2): 1-17.
10. DOLGHI A, et al. Chemical and antimicrobial characterization of Mentha piperita L. and Rosmarinus officinalis L. essential oils and in vitro potential cytotoxic effect in human colorectal carcinoma cells. Molecules, 2022; 27(18): 6106.
11. EJAZ R, et al. Anti-biofilm potential of menthol purified from Mentha piperitaL. (Mint). Biological and Clinical Sciences Research Journal, 2020; 1.
12. EL-HOSSARY D, et al. Antibiotic Resistance, Virulence Gene Detection, and Biofilm Formation in Aeromonas spp. Isolated from Fish and Humans in Egypt. Biology, 2023; 12(3): 421.
13. FAO. FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS. In: Food and Agriculture Organization of the United Nations (FAO), 2020.
14. FRANCISCONI R, et al. Antibio film efficacy of tea tree oil and of its main component terpinen-4-ol Against Candida albicans. Brazilian oral research, 2020; 34.
15. FUJIMOTO R, et al. Controle alternativo de helmintos de Astyanax cf. zonatus utilizando fitoterapia com sementes de abóbora (Cucurbita maxima) e mamão (Caricapapaya). Pesquisa Veterinária Brasileira, 2012; 32: 5-10.
16. GALLANI SU, et al. Motile Aeromonas septicemia in tambaqui Colossoma macropomum: Pathogenicity, lethality and new insights for controland disinfection in aquaculture. Microbial Pathogenesis, 2020; 149: 104512.
17. GASTALHO S, et al. Uso de antibióticos em aquacultura e resistência bacteriana: Impacto em saúde pública. Acta Farmacêutica Portuguesa, [s.l.] centro de Estudos Farmacêuticos, Faculdade de Farmácia da Universidade de Coimbra, 2014; 3(1): 29-45.
18. GROOT AC e SCHMIDT E. Tea tree oil: contact allergy and Chemical composition. Contact dermatitis, 2016; 75(3): 129-143.
19. HERNÁNDEZ F, et al. Antibiotic residue determination in environment al waters by LC-MS. TrAC Trends in Analytical Chemistry, 2007; 26(6): 466-485.
20. HILSDORF A, et al. The farming and husban dry of Colossoma macropomum: From Amazonian Waters to sustainable production. Reviews in Aquaculture, 2022; 14(2): 993-1027.
21. HU M, et al. Identity and virulence Properties of Aeromonas isolates from diseased fish, healthy controls and water environment in China. Letters in Applied microbiology, 2012; 55(3): 224-233.
22. HUSSAIN AI, et al. Season alvariation in content, Chemical composition and antimicrobial and cytotoxic activities of essential oils from four Menthaspecies. Journal of the Science of Food and Agriculture, 2010; 90(11): 1827- 1836.
23. IGBINOSA I, et al. Emerging Aeromonas species infections and their significance in publichealth. The Scientific World Journal, 2012.
24. KAIREY L, et al. Efficacy and safety of Melaleuca alternifolia (teatree) oil for human health—A systematic review of randomized controlled trials. Frontiers in pharmacology, 2023; 14: 1116077.
25. KUBITZA F. Os caminhos para uma piscicultura sustentável. Panorama da Aquicultura, 2010; 20(119): 16-23.
26. KULKARNI R, et al. Lavandula gibsoni and Plectranthusmollis essential oils: Chemical analysis and insect control activities against Aedes aegypti, Anopheles sfttephensiand Culex quinquefasciatus. Journal Of Pest Science, [s.l.], Springer Nature, 2013; 86(4): 713-718.
27. KUMAR S, et al. Effect of orally administeredaza dirachtinon non-specificimmune parameters of gold fish Carassiusauratus (Linn. 1758) and resistance Against Aeromonashydrophila. Fish & shell fish immunology, 2013; 34(2): 564-573.
28. LAMY B, et al. Accuracyof 6 commercial systems for identifying clinical Aeromonas isolates. Diagnostic microbiology and infectious disease, 2010; 67(1): 9-14.
29. LEITE MC e CROZARAMA. Fitoterapia e o uso indiscriminado do chá de Cannabis sativa. Simpósio. São Camilo: 2015; 1- 3.
30. LIMA ROHA. Atividade antimicrobiana de óleos essenciais frente a Aeromonas spp. isoladas do peixe amazônico tambaqui (Colossoma macropomum), 2019.
31. MARQUES DSC, et al. Impact of stress on Aeromonasdiversity in tambaqui (Colossomamacropomum) and lectin level change to wards a bacterial challenge. Environmental technology, 2016; 37(233030-3035).
32. MARQUES DSC. Isolamento e Identificação Taxonômica de Aeromonas sp. em Tambaquis (Colossoma macropomum) e Análise do Perfil de Lectinas Séricas Frente a um Desafio com os Isolados Endógenos, 2015.
33. MOGOSAN C, et al. A comparative analysis of the Chemical composition, anti-inflammatory, and antinociceptive effects of the essential oils from thre espécies of Menth acultivated in Romania. Molecules, 2017; 22(2): 263.
34. PESSOA RBG, et al. Aeromonas and human health disorders: Clinical approaches. Frontiers in microbiology, 2022; 13: 868890.
35. PESSOA RBG, et al. Fluorescent nanoprobes based on quantum dots conjugated to Cramollto assess surface carbo hydrates of Aeromonas spp. Biochimica et Biophysica Acta (BBA)-General Subjects, 2023; 1867(7): 130373.
36. PESSOA RBG, et al. Molecular characterization and evaluation of virulence traits of Aeromonas spp. Isolated from the tambaqui fish (Colossoma macropomum). Microbial pathogenesis, 2020; 147: 104273.
37. PESSOA RBG, et al. The genus Aeromonas: A general approach. Microbial Pathogenesis, [s.l.], 2019; 130: 81-94.
38. RHIND J. Essential Oils: A Comprehensive Handbook for Aromatic Therapy. Singing Dragon, 2020.
39. SAHA D e PAL J. In vitro antibiotic susceptibility of bactéria isolated from EUS‐affected fishes in India. Letters in Applied Microbiology, 2002; 34(5): 311-316.
40. SOUSA RGC, et al. Avaliação do ganho do peso de tambaqui cultivado com diferentes taxas de proteínas na alimentação. Biota Amazônia (Biote Amazonie, Biota Amazonia, Amazonian Biota), 2016; 6(1): 40-45.
41. STEPANOVIĆ S, et al. A modified microtiter-platetest for quantification of staphylococcal biofilm formation. Journal of microbiological methods, 2000; 40(2): 175-179.
42. STRINGARO A, et al. Antioxidant, Antifungal, Antibiofilm, and Cytotoxic Activities of Mentha spp. Essential Oils. Medicines, 2018; 5(4): 112.
43. TAVARES-DIAS M e MARIANOWS. Aquicultura no Brasil: novas perspectivas. São Carlos: Pedro & João Editores, 2015.