Associação de melatonina, própolis verde e paclitaxel promove inibição de tumores de Ehrlich em camundongos
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Jul 30, 2025
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Resumo
Objetivo: Avaliar a ação antitumoral da Própolis Verde e da Melatonina. Métodos: Células de carcinoma de Ehrlich foram implantadas no tecido subcutâneo de camundongos Swiss Webster machos, os quais foram divididos nos grupos experimentais: Controle, Animais que receberam 2 mg/kg de Melatonina; Animais tratados com 50 mg/Kg de Própolis Verde; Animais tratados com 5 mg/Kg de Paclitaxel; Animais tratados com Melatonina e Própolis Verde; Animais tratados com Melatonina e Paclitaxel; Animais tratados com Própolis Verde e Paclitaxel; e Animais tratados com Melatonina, Própolis Verde e Paclitaxel. Resultados: Todos os protocolos contendo Própolis Verde preveniram lesões metastáticas e degenerativas no pulmão e no fígado, minimizando os efeitos colaterais da poliquimioterapia. Embora nas condições aplicadas neste estudo, a Melatonina não tenha sido eficiente em inibir o EST, ela atenuou a miocardite, a necrose tubular renal e a metástase pulmonar. Conclusão: O tratamento com Melatonina, Própolis Verde e Paclitaxel apresentou os melhores resultados na redução do peso tumoral, índice mitótico e inibição tumoral, reforçando a eficácia da associação de compostos naturais com a quimioterapia na redução das alterações histopatológicas teciduais.
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Como Citar
PintoC. F., DiasF. C. R., TorresS. M., MoraisD. B., PereiraM. de F., & Silva JúniorV. A. da. (2025). Associação de melatonina, própolis verde e paclitaxel promove inibição de tumores de Ehrlich em camundongos. Revista Eletrônica Acervo Saúde, 25(7), e20981. https://doi.org/10.25248/reas.e20981.2025
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Referências
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2. ALBALAWI AE, et al. Antitumor activity of royal jelly and its cellular mechanisms against Ehrlich solid tumor in mice. BioMed Research International, 2022; 2022.
3. ALI BH, et al. Effect of concomitant treatment of curcumin and melatonin on cisplatin-induced nephrotoxicity in rats. Biomedicine and Pharmacotherapy, 2020; 131.
4. ALMEER RS, et al. Effects of green cardamom (Elettaria cardamomum Maton) and its combination with cyclophosphamide on Ehrlich solid tumors. BMC Complementary Medicine and Therapies, 2021; 21.
5. AMIN AH, et al. Ameliorative effects of melatonin against solid Ehrlich carcinoma progression in female mice. Journal of Pineal Research, 2019; 67.
6. ANSELMO J, et al. Melatonina e câncer - revisão da literatura.
7. BARTSCH CBHKM. Melatonin in clinical oncology. Neuro Endocrinology Letters, 2002; 23: 30–38.
8. BENKOVIC V, et al. Enhanced antitumor activity of irinotecan combined with propolis and its polyphenolic compounds on Ehrlich ascites tumor in mice. Biomedicine and Pharmacotherapy, 2007; 61: 292–297.
9. CARDOSO EO, et al. Brazilian green propolis: A novel tool to improve the cytotoxic and immunomodulatory action of docetaxel on MCF-7 breast cancer cells and on women monocyte. Phytother Res, 2022; 36: 448–461.
10. CHANG JF, et al. Therapeutic targeting of aristolochic acid induced uremic toxin retention, SMAD 2/3 and JNK/ERK pathways in tubulointerstitial fibrosis: Nephroprotective role of propolis in chronic kidney disease. Toxins, 2020; 12.
11. CHAO CC, et al. Melatonin suppresses lung cancer metastasis by inhibition of epithelial-mesenchymal transition through targeting to Twist. Clinical Science, 2019; 133: 709–722.
12. CHIU HF, et al. Chiu 2020. Journal of Cancer Prevention, 2020; 25: 70–78.
13. COLES EH. Patologia Clínica Veterinária. 3 ed. São Paulo: Manole, 1984; 345p.
14. DAIR EL, et al. Effects of melatonin on the endometrial morphology and embryo implantation in rats. Fertility and Sterility, 2008; 89: 1299–1305.
15. DIAS MBMC, et al. Perfil hematológico e bioquímico de camundongos com carcinoma de Ehrlich tratados com cisplatina, acupuntura e Euphorbia tirucalli L. (avelóz). Revista Brasileira de Terapias e Saúde, 2017; 8: 7–14.
16. ELGHARABAWY RM, et al. Therapeutic impact of Costus (Saussurea lappa) against Ehrlich solid tumor-induced cardiac toxicity and DNA damage in female mice. Frontiers in Pharmacology, 2021; 12.
17. EL-GUENDOUZ S, et al. Anti-acetylcholinesterase, antidiabetic, anti-inflammatory, antityrosinase and antixanthine oxidase activities of Moroccan propolis. International Journal of Food Science and Technology, 2016; 51: 1762–1773.
18. EL-KHAWAGA OAY, et al. Protective role of Egyptian propolis against tumor in mice. Clinica Chimica Acta, 2003; 338: 11–16.
19. EL-MISSIRY MA, ABD EL-AZIZ AF. Influence of melatonin on proliferation and antioxidant system in Ehrlich ascites carcinoma cells.
20. GHONEUM M, et al. Dietary baker’s yeast sensitizes Ehrlich mammary adenocarcinoma to paclitaxel in mice bearing tumor. Oncology Reports, 2019; 41: 3155–3166.
21. GONZALEZ-GRONOW M, et al. Glucose-regulated protein (GRP78) is an important cell surface receptor for viral invasion, cancers, and neurological disorders. IUBMB Life, 2021; 73: 843–854.
22. JOSÉ M, et al. Efeito do tratamento com própolis de Scaptotrigona aff. postica sobre o desenvolvimento do tumor de Ehrlich em camundongos.
23. KARBOWNIK M, REITER RJ. Antioxidative effects of melatonin in protection against cellular damage caused by ionizing radiation. Proc Soc Exp Biol Med, 2000; 225: 9–22.
24. KOCOT J, et al. Antioxidant potential of propolis, bee pollen, and royal jelly: Possible medical application. Oxidative Medicine and Cellular Longevity, 2018; 2018.
25. LIN PH, et al. Melatonin activates cell death programs for the suppression of uterine leiomyoma cell proliferation. Journal of Pineal Research, 2020; 68.
26. MALEKI DANA P, et al. Melatonin as a potential inhibitor of kidney cancer: A survey of the molecular processes. IUBMB Life, 2020; 72: 2355–2365.
27. MANGUEIRA VM, et al. A new acridine derivative induces cell cycle arrest and antiangiogenic effect on Ehrlich ascites carcinoma model. Biomedicine and Pharmacotherapy, 2017; 90: 253–261.
28. MUNARI CC, et al. Mutagenicity and antimutagenicity of Baccharis dracunculifolia extract in chromosomal aberration assays in Chinese hamster ovary cells. Planta Medica, 2008; 74: 1363–1370.
29. ORŠOLIĆ N, BAŠIĆ I. Water-soluble derivative of propolis and its polyphenolic compounds enhance tumoricidal activity of macrophages. Journal of Ethnopharmacology, 2005; 102: 37–45.
30. ORŠOLIĆ N, et al. Protective effects of propolis and related polyphenolic/flavonoid compounds against toxicity induced by irinotecan. Medical Oncology, 2010; 27: 1346–1358.
31. POURHANIFEH MH, et al. Melatonin and non-small cell lung cancer: New insights into signaling pathways. Cancer Cell International, 2019; 19.
32. SAMENI HR, et al. Co-administration of 5FU and propolis on AOM/DSS induced colorectal cancer in BALB-c mice. Life Sciences, 2021; 276.
33. SEGURA JA, et al. Early tumor effect on splenic Th lymphocytes in mice. FEBS Letters, 1997; 414: 1–6.
34. SHARAWI ZW. Therapeutic effect of Arthrocnemum machrostachyum methanolic extract on Ehrlich solid tumor in mice. BMC Complementary Medicine and Therapies, 2020; 20.
35. SIMBRE II VC, et al. Cardiotoxicity of cancer chemotherapy: Implications for children.
36. SOUSA WO, et al. Effects of modified melatonin release on human colostrum neutrophils to induce death in the MCF-7 cell line. International Journal of Cell Biology, 2022; 2022.
37. SUZUKI I, et al. Antitumor and anticytopenic effects of aqueous extracts of propolis in combination with chemotherapeutic agents.
38. TALIB WH, et al. Melatonin in cancer treatment: Current knowledge and future opportunities. Molecules, 2021; 26.
39. TSIMBERIDOU AM, et al. Review of precision cancer medicine: Evolution of the treatment paradigm. Cancer Treatment Reviews, 2020; 86.
2. ALBALAWI AE, et al. Antitumor activity of royal jelly and its cellular mechanisms against Ehrlich solid tumor in mice. BioMed Research International, 2022; 2022.
3. ALI BH, et al. Effect of concomitant treatment of curcumin and melatonin on cisplatin-induced nephrotoxicity in rats. Biomedicine and Pharmacotherapy, 2020; 131.
4. ALMEER RS, et al. Effects of green cardamom (Elettaria cardamomum Maton) and its combination with cyclophosphamide on Ehrlich solid tumors. BMC Complementary Medicine and Therapies, 2021; 21.
5. AMIN AH, et al. Ameliorative effects of melatonin against solid Ehrlich carcinoma progression in female mice. Journal of Pineal Research, 2019; 67.
6. ANSELMO J, et al. Melatonina e câncer - revisão da literatura.
7. BARTSCH CBHKM. Melatonin in clinical oncology. Neuro Endocrinology Letters, 2002; 23: 30–38.
8. BENKOVIC V, et al. Enhanced antitumor activity of irinotecan combined with propolis and its polyphenolic compounds on Ehrlich ascites tumor in mice. Biomedicine and Pharmacotherapy, 2007; 61: 292–297.
9. CARDOSO EO, et al. Brazilian green propolis: A novel tool to improve the cytotoxic and immunomodulatory action of docetaxel on MCF-7 breast cancer cells and on women monocyte. Phytother Res, 2022; 36: 448–461.
10. CHANG JF, et al. Therapeutic targeting of aristolochic acid induced uremic toxin retention, SMAD 2/3 and JNK/ERK pathways in tubulointerstitial fibrosis: Nephroprotective role of propolis in chronic kidney disease. Toxins, 2020; 12.
11. CHAO CC, et al. Melatonin suppresses lung cancer metastasis by inhibition of epithelial-mesenchymal transition through targeting to Twist. Clinical Science, 2019; 133: 709–722.
12. CHIU HF, et al. Chiu 2020. Journal of Cancer Prevention, 2020; 25: 70–78.
13. COLES EH. Patologia Clínica Veterinária. 3 ed. São Paulo: Manole, 1984; 345p.
14. DAIR EL, et al. Effects of melatonin on the endometrial morphology and embryo implantation in rats. Fertility and Sterility, 2008; 89: 1299–1305.
15. DIAS MBMC, et al. Perfil hematológico e bioquímico de camundongos com carcinoma de Ehrlich tratados com cisplatina, acupuntura e Euphorbia tirucalli L. (avelóz). Revista Brasileira de Terapias e Saúde, 2017; 8: 7–14.
16. ELGHARABAWY RM, et al. Therapeutic impact of Costus (Saussurea lappa) against Ehrlich solid tumor-induced cardiac toxicity and DNA damage in female mice. Frontiers in Pharmacology, 2021; 12.
17. EL-GUENDOUZ S, et al. Anti-acetylcholinesterase, antidiabetic, anti-inflammatory, antityrosinase and antixanthine oxidase activities of Moroccan propolis. International Journal of Food Science and Technology, 2016; 51: 1762–1773.
18. EL-KHAWAGA OAY, et al. Protective role of Egyptian propolis against tumor in mice. Clinica Chimica Acta, 2003; 338: 11–16.
19. EL-MISSIRY MA, ABD EL-AZIZ AF. Influence of melatonin on proliferation and antioxidant system in Ehrlich ascites carcinoma cells.
20. GHONEUM M, et al. Dietary baker’s yeast sensitizes Ehrlich mammary adenocarcinoma to paclitaxel in mice bearing tumor. Oncology Reports, 2019; 41: 3155–3166.
21. GONZALEZ-GRONOW M, et al. Glucose-regulated protein (GRP78) is an important cell surface receptor for viral invasion, cancers, and neurological disorders. IUBMB Life, 2021; 73: 843–854.
22. JOSÉ M, et al. Efeito do tratamento com própolis de Scaptotrigona aff. postica sobre o desenvolvimento do tumor de Ehrlich em camundongos.
23. KARBOWNIK M, REITER RJ. Antioxidative effects of melatonin in protection against cellular damage caused by ionizing radiation. Proc Soc Exp Biol Med, 2000; 225: 9–22.
24. KOCOT J, et al. Antioxidant potential of propolis, bee pollen, and royal jelly: Possible medical application. Oxidative Medicine and Cellular Longevity, 2018; 2018.
25. LIN PH, et al. Melatonin activates cell death programs for the suppression of uterine leiomyoma cell proliferation. Journal of Pineal Research, 2020; 68.
26. MALEKI DANA P, et al. Melatonin as a potential inhibitor of kidney cancer: A survey of the molecular processes. IUBMB Life, 2020; 72: 2355–2365.
27. MANGUEIRA VM, et al. A new acridine derivative induces cell cycle arrest and antiangiogenic effect on Ehrlich ascites carcinoma model. Biomedicine and Pharmacotherapy, 2017; 90: 253–261.
28. MUNARI CC, et al. Mutagenicity and antimutagenicity of Baccharis dracunculifolia extract in chromosomal aberration assays in Chinese hamster ovary cells. Planta Medica, 2008; 74: 1363–1370.
29. ORŠOLIĆ N, BAŠIĆ I. Water-soluble derivative of propolis and its polyphenolic compounds enhance tumoricidal activity of macrophages. Journal of Ethnopharmacology, 2005; 102: 37–45.
30. ORŠOLIĆ N, et al. Protective effects of propolis and related polyphenolic/flavonoid compounds against toxicity induced by irinotecan. Medical Oncology, 2010; 27: 1346–1358.
31. POURHANIFEH MH, et al. Melatonin and non-small cell lung cancer: New insights into signaling pathways. Cancer Cell International, 2019; 19.
32. SAMENI HR, et al. Co-administration of 5FU and propolis on AOM/DSS induced colorectal cancer in BALB-c mice. Life Sciences, 2021; 276.
33. SEGURA JA, et al. Early tumor effect on splenic Th lymphocytes in mice. FEBS Letters, 1997; 414: 1–6.
34. SHARAWI ZW. Therapeutic effect of Arthrocnemum machrostachyum methanolic extract on Ehrlich solid tumor in mice. BMC Complementary Medicine and Therapies, 2020; 20.
35. SIMBRE II VC, et al. Cardiotoxicity of cancer chemotherapy: Implications for children.
36. SOUSA WO, et al. Effects of modified melatonin release on human colostrum neutrophils to induce death in the MCF-7 cell line. International Journal of Cell Biology, 2022; 2022.
37. SUZUKI I, et al. Antitumor and anticytopenic effects of aqueous extracts of propolis in combination with chemotherapeutic agents.
38. TALIB WH, et al. Melatonin in cancer treatment: Current knowledge and future opportunities. Molecules, 2021; 26.
39. TSIMBERIDOU AM, et al. Review of precision cancer medicine: Evolution of the treatment paradigm. Cancer Treatment Reviews, 2020; 86.